Figure 4.

X-ray images of joint tissue destruction in the ankle- and lumbar-spine vertebrae of collagen-injected CA1-Tg mice. Evident bone formation (indicated with an arrow) was observed in the hind paws of immunized CA1-Tg mice (n = 5) (A) but not in the paws of wild-type mice (n = 5) (B), PADI4-Tg mice (n = 5) (C) or BSA-treated CA1-Tg mice (n = 5) (D). The disk space disappeared, and a chondrophyte/osteophyte was fused with the neighboring vertebral bodies between L5 and S1 (E) as well as S4 and S5 (I). There was no apparent bone fusion in the lumbar spine vertebrae of collagen-injected wild-type mice (F, J), PADI4-Tg mice (G, K) or BSA-treated CA1-Tg mice (H, L).