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. 2012 Oct 30;12:201. doi: 10.1186/1472-6882-12-201

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Copyright ©2012 Lin et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of EGCG on EMT phenotype. Mesenchymal cell marker, vimentin expression was assayed by immunofluorescence staining in TW01 sphere-derived cells (A~C). (A) control group (B) treated with 20 μM EGCG (C) treated with 40 μM EGCG. EGCG could decrease the expression of vimentin. Green fluorescent signal represents vimentin expression. Blue color is DAPI stain for nucleus. (D) Western blot signals in TW01 sphere-derived cells show decreased expression of vimentin and snail and increased expression of E-cadh (e-cadherin) after EGCG treatment.