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. 2012 Oct 30;12:201. doi: 10.1186/1472-6882-12-201

Table 1.

The primer sequences for q-RT PCR used in this study

Gene Sequence
GAPDH
Forward : 5-ACGGGAAGCTCACTGGCATGG-3
Reverse : 5-GGTCCACCACCCTGTTGCTGTA-3
Sox-2
Forward : 5-CGAGTGGAAACTTTTGTCGGA-3
Reverse: 5-TGTGCAGCGCTCGCAG-3
Oct-4
Forward: 5-GTGGAGAGCAACTCCGATG-3
Reverse: 5-TGCTCCAGCTTCTCCTTCTC-3
c-Myc
Forward: 5-GGAACGAGCTAAAACGGAGCT-3
Reverse: 5-GGCCTTTTCATTGTTTTCCAACT-3
Klf-4
Forward: 5-CCGCTCCATTACCAAGAGCT-3
Reverse: 5-ATCGTCTTCCCCTCTTTGGC-3
E-Cad
Forward: 5-TGCCCAGAAAATGAAAAAGG-3
Reverse: 5-GTGTATGTGGCAATGCGTTC-3
Vim
Forward: 5-GAGAACTTTGCCGTTGAAGC-3
Reverse: 5-GCTTCCTGTAGGTGGCAATC-3
Snail
Forward: 5-CTTCCAGCAGCCCTACGAC-3
Reverse: 5-CGGTGGGGTTGAGGATCT-3
N-Cad
Forward: 5-AGGGTGGACGTCATTGTAGC-3
Reverse: 5-CTGTTGGGGTCTGTCAGGAT-3
Twist Forward: 5-CGAGTCCGCAGTCTTACGAG-3
Reverse: 5-TCTGGAGGACCTGGTAGAGG-3