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. 2013 Feb 18;8(2):e55979. doi: 10.1371/journal.pone.0055979

Figure 2. LPS-stimulated PKCδ activation in MCD diet mice.

Figure 2

(A) Liver sections from ND mice and LPS-injected MCD mice were analyzed for E-cadherin, α-SMA and phospho-PKCδ expression using immunohistochemistry staining (original magnification 200×). (B) Liver tissue extracts were prepared from mice fed MCD diet for 1 weeks or 3 weeks and subsequently injected with 2.5 mg/kg LPS. Liver phospho-PKCδ protein contents were quantified by Western blot using an equal amount of total liver proteins. Expression levels were normalized relative to GAPDH. Liver extracts from a pool of three mice in each group were analyzed by Western blot using specific antibodies.