Table 2.
Cellsβ | GJICα | |
---|---|---|
Adenosquamous carcinoma, moderately differentiated |
carcinoma cells |
0.1 ±0.1 |
|
fibroblasts |
5.8±1.2 |
Adenocarcinoma, poorly differentiated |
carcinoma cells |
0.1 ±0.1 |
Adenocarcinoma | carcinoma cells | 0.1 ±0.1 |
αImmediately after surgery, cells were placed in culture and GJIC examined (see Methods, Figure 7). After 8-10 weeks in culture, most of the tumor cells had died while the fibroblasts present in the initial suspension predominated. These cells did not express cytokeratins, contrary to tumor cells [18]. The fibroblasts shown were derived from the moderately differentiated adenosquamous carcinoma tumor above (Figure 3, c-d). GJIC was examined as in Table 1, at 3 days after confluence.