Table 1. Bacterial strains, protozoa and plasmids used in this study.

Strain/Plasmid	Properties	Reference or origin
Bacterial strains
V. cholerae A1552	O1 El Tor, Inaba, Rifr	[15]
V. cholerae vpsA	A1552, ΔvpsA	[15]
V. cholerae vpsL	A1552, ΔvpsL	[15]
V. cholerae hapR	A1552, hapR::pGP704, Apr	[47]
V. cholerae vpsA/hapR	A1552, ΔvpsA hapR::pGP704-cat,Cmr	This study
V. cholerae vpsL/hapR	A1552, ΔvpsL hapR::pGP704-cat,Cmr	This study
V. cholerae A1552 GFP	A1552, mTn7-GFP, Gmr	[53]
V. cholerae hapR RFP	hapR, mTn10-DsRedExpress, Apr Kmr Cmr	This study
E. coli S17-1 λpir	recA thi pro rK - mK + RP4::2-Tc::MuKm Tn7 Tpr Smr λpir	[54]
Protozoa
Rhynchonomas nasuta	Wild Type	[14]
Acanthamoeba castellanii	Wild Type	ATCC 30234
Plasmids
pGP704.hapR.cat	pGP704 [55] containing 0.3 kb internal region of hapR disrupted by 0.9 kb cat from pLG401(Lynn Gilson, University of Hawaii)	This study
pKKS07	Kmr, Apr, Cmr, pBLS180 [56] containing dsred and cat from pBK-miniTn7dsred [57]	Krager Koh, Nanyang Technological University