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. 2013 Feb 18;8(2):e56316. doi: 10.1371/journal.pone.0056316

Figure 2. Selective activation of p38α during anti- GBM nephritis.

Figure 2

(A) p38MAPK phosphorylation during anti-GBM induced nephritis was investigated at early (35 min) and late (14 days) time points after disease induction using phospho-specific antibodies (left graph). Control lane shows no p38 phosphorylation before injection of anti- GBM antibodies. (B) Co-Immunoprecipitation (IP) was used to determine which p38 isoform is activated during anti- GBM nephritis. Kidney tissue was retrieved three days after induction of glomerulonephritis. IP was performed with buffer only (−) or with kidney lysates and anti-phospho p38MAPK antibody, or with kidney lysates using an isotype-matched control antibody (Iso). IPs and positive control lysate (+) were separated by SDS-PAGE, blotted onto nitrocellulose and probed with specific antibodies against p38 isoforms (arrows). HC: heavy chain of the precipitating antibody (right graph).