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. 2013 Feb 18;8(2):e55714. doi: 10.1371/journal.pone.0055714

Figure 3. SPC-A-1 sci cells transfected with siRNA-osteopontin, LAMB3 and ITGB1 display low migration and invasion in vitro.

Figure 3

(A) RT-PCR was performed and showed effective knock-down of expression mediated by siRNA-osteopontin, siRNA-LAMB3 and siRNA-ITGB1. (B) Western blot showed decreased expression of osteopontin, LAMB3 and ITGB1. (C and D) Migration and invasion assays of SPC-A-1sci, SPC-A1sci/siRNA-osteopontin, SPC-A-1sci/siRNA-LAMB3 and SPC-A1sci/siRNA-ITGB1 (400× magnification). Three independent experiments were performed; results are expressed as the mean +SD; *P<0.05, **P<0.01, ***P<0.001.