Figure 4.

The HLA class I allele calls from the first genotyping experiment, designed to test the use of the two-tiled class I amplicons, are shown. Calls in blue were completely unambiguous to the allele level. Calls in green were four-digit ambiguous based on distinguishing SNPs outside the amplified region. The call in orange was only recovered using the modified, lower-stringency analysis pipeline, while the call in pink was expected but not observed for both amplicons. Calls in purple were examples of reference typing undergoing correction, or the detection of a putative novel allele.