Figure 5. CADs that strongly inhibit EBOV entry and infection cause cholesterol accumulation in LE/Lys.

SNB19 cells were treated for 21 hr with either DMSO or inhibitor (concentrations as in Fig. 2). Cells were then fixed, stained with filipin, and imaged on a fluorescence microscope. Images were inverted and uniformly adjusted for contrast and brightness. Representative images are shown. Arrows indicate sites of cholesterol accumulation. Each compound was tested at least 3 times, and scored (+/−) by a blind observer (Table 1).