Figure 10. Ethanol does not promote apoptotic cell death. RFP(+)-MCF7 cells were plated in homotypic culture or in co-culture with fibroblasts. Cells were treated with 100 mM EtOH for 72 h. Apoptosis was detected with annexin V-APC and PI by flow cytometry. Note that MCF7 cells are protected against cell death when co-cultured with fibroblasts (A). However, ethanol does not significantly promote apoptosis in MCT7 cells (A) or in fibroblasts (B).