Figure 12. Antioxidant treatment prevents the ethanol-induced upregulation of MCT4 in cancer-associated fibroblasts. MCF7 cells were plated in co-culture with fibroblasts, and cells were treated with 100 mM EtOH for 72 h. Then, 10 mM NAC or vehicle was added for 24 h. Cells were fixed and immunostained with antibodies against MCT4 (red, upper panels) and K8/18 (green, lower panels). Nuclei were counterstained with DAPI (blue). Note that treatment with the antioxidant NAC prevents the upregulation of MCT4 in fibroblasts compartment, as compared with co-cultured fibroblasts in the absence of NAC. Original magnification, 40x.