Figure 9. Ethanol suppresses the expression of ERα in MCF7 cancer cells. MCF7 cell-fibroblast co-cultures were treated with 100 mM EtOH for 72 h. Cells were fixed and immunostained with antibody probes directed against ERα (red) and K8/18 (green). Nuclei were counterstained with DAPI (blue). The upper panels show ERα staining only (red). The bottom panels show also the K8/18 staining (green) to identify the MCF7 cell population. Note that ethanol suppresses the expression of ERα specifically in MCF7 cells, as compared with untreated cells. Original magnification, 40x.