Figure 11. Replication of KY/180E and KY/136E in mouse macrophage cell lines.

Mouse macrophage cell line, RAW264.7, was infected in vitro with the influenza isolates on a 24-well plate at an MOI = 1.0. After one hour of incubation, the cells were washed and returned to the incubator. Cell culture supernatants were collected over time and virus titer was determined by TCID₅₀ assay. Both KY/180E and KY/136E replicate in mouse macrophage cell lines. KY/180E had significantly higher titers of virus detected at 24 and 48 hours post-infection (p<0.05, indicated by asterisks on the figure). Pathogenic isolate KY/180E was able to replicate better in mouse macrophages compared to the low pathogenic isolate, KY/136E.