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. 2013 Feb 18;8(2):e57090. doi: 10.1371/journal.pone.0057090

Figure 3. C. trachomatis transformants expressing RSGFP, mCherry or CFP from the incD gene promoter.

Figure 3

A–C Maps of the p2TK-SW2 derivatives allowing expression of RSGFP (A), mCherry (B) or CFP (C) from the incDEFG operon promoter. The pSW2 plasmid is shown in black, the E.coli origin of replication in dark grey and the ampicillin resistance cassette (bla) in light grey. The rsgfp (green), mCherry (red) and cfp (blue) ORFs are flanked by the incDEFG operon promoter and terminator (light grey). Unique restriction sites are shown in red. D–F. Immunofluorescence images of C. trachomatis inclusions harboring bacteria expressing RSGFP (D), mCherry (E) or CFP (F) from the incDEFG operon promoter. Scale Bar: 10 µm.