Table 1. Degenerative primers used for the isolation of crustacyanin subunit A and C.
Primer name | Sequence 5' → 3' * | Crustacyanin |
A1F | TAY CAR CCN TAY AC | subunit A |
A2F | GGN AAR ATH TAY CCN ACN AAN GAY TTY CC | subunit A |
A2R | GGR AAR TCN TTN GTN GGR TAD ATY TTN CC | subunit A |
A3R | AAN ACR AAN CCR AAY TC | subunit A |
A4R | GTR TCR TAR TCN GTN TC | subunit A |
C1F | CCN AAY CCN TTY GGN GAR CCN CA | subunit C |
C1R | TGN GGY TCN CCR AAN GGR TTN GG | subunit C |
C2R | GAY ACN GAY TAY GAR AA | subunit C |
N = A, C, T or G; Y = C or T; R = A or G; H = A, C or T; D = A, G or T.