Table 2. Primer pairs for qPCR analysis of dark, light and albino F. merguiensis individuals.
Primer name | Primer sequence (5' → 3') | Tm (°C) | Amplicon size (bp) | Crustacyanin | Source sequence | Efficiency |
SubA2F | CAG GGC AAG ATC TAC CCC ACA | 64.5 | 175 | subunit A | HM370278 | 0.957 |
SubA2R | GGG AGA ACA CGA AGC CGA AC | 64.3 | ||||
SubC1F | TGC TGG CAC ATG GTA CGA AA | 63.6 | 205 | subunit C | HM370279 | 0.939 |
SubC1R | TGC CTC GTA GTC CAC GGA AA | 64 |