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. 2013 Feb 18;8(2):e56920. doi: 10.1371/journal.pone.0056920

Table 2. Primer pairs for qPCR analysis of dark, light and albino F. merguiensis individuals.

Primer name Primer sequence (5' → 3') Tm (°C) Amplicon size (bp) Crustacyanin Source sequence Efficiency
SubA2F CAG GGC AAG ATC TAC CCC ACA 64.5 175 subunit A HM370278 0.957
SubA2R GGG AGA ACA CGA AGC CGA AC 64.3
SubC1F TGC TGG CAC ATG GTA CGA AA 63.6 205 subunit C HM370279 0.939
SubC1R TGC CTC GTA GTC CAC GGA AA 64