Figure 3. Demethylation induces Oxtr expression in 4T1 cells.
A) In GT1-7 cells, which normally show high Oxtr mRNA levels and low methylation, A two-day treatment with 5-AzaC caused a slight reduction in Oxtr mRNA levels B)Conversely, treatment of 4T1 cells with 5-AzaC caused a robust dose-dependent increase in Oxtr mRNA levels.. C) 5-AzaC treatment (2 µM) of 4T1 cells caused a significant reduction in the methylation of the Oxtr promoter (compared to vehicle samples, also shown in Fig. 2B) across all seven CpG sites of the amplicon. Statistics: A) One-way ANOVA F(3) = 90.1, p<0.001, * differ from vehicle-treated, † differ from 0.5 µM, p<0.05, n = 5. B) One-way ANOVA F(3) = 39.6, p<0.001, * differ from vehicle-treated, p<0.01, n = 5.