Table 1. PCR primer sets.

Primers for amplification of:	Sequence	TA
Unmodified vector	F: 5′-GGCCATTAATTTAATTAACCTTATTTTACAGATGG-3′ R: 5′-GGAAGGATCCACTAGTAGATTGGGAAAGCTGCTG-3′	59°C, 30 s
Mut 1 vector	F: 5′-GTTCCTTTTGGTCCTAGGTCATTGTAAATCAG-3′ R: 5′-CTGATTTACAATGACCTAGGACCAAAAGGAAC-3′	60°C, 30 s
Mut 7 vector	F: 5′-GGACTGGGGGTGGGGAGGGGATACAGGGTGTG-3′ R: 5′-CACACCCTGTATCCCCTCCCCACCCCCAGTCC-3′	60°C, 30 s
Del vector	F: 5′-GGGAATTCCTCTCAAGGCTGGTGGGAG-3′ R: 5′-GGGAATTCGCTCTCTAACGTGATTA-3′	60°C. 30 s
Oxtr mRNA	F: 5′-GTGCAGATGTGGAGCGTCT-3′ R: 5′-GTTGAGGCTGGCCAAGAG-3′	60°C, 30 s
Hprt1 mRNA	F: 5′-GGGATTTGAATCACGTTTGTG-3′ R: 5′-TTGCGCTCATCTTAGGCTTT-3′	60°C, 30 s
Bisulfite treated Oxtr First PCR round	5′- TTGGTTTTTTTTGTTTTTTTTG-3′ 5′- AAAAAATATCTACCCCTCCCA-3′	54°C, 30 s
Bisulfite treated Oxtr Second PCR round (Nested PCR)	5′- TTGGTTTTTTTTGTTTTTTTTG-3′ 5′- CCACCAACCTTAAAAAAAATCT-3′	54°C, 30 s

T_A, Temperatures and time applied for annealing.