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. 2013 Feb 18;8(2):e57002. doi: 10.1371/journal.pone.0057002

Figure 1. Detection of HIV gag RNA in situ in cell lines and PBMCs.

Figure 1

(a) Representative color merged 60x images of the HIV+ cell line H9IIIB (left) and HIV negative cell line H9 (right); HIV gag RNA-Alexa Fluor® 647 (green), 18 s rRNA-FITC (red), and Nuclei-DAPI (blue). (b) The frequency of HIV gag RNA-positive cells within the 18 s+DAPI+ population was calculated after spiking HIV+ cells into the negative cell population at the stated percentages in the table. The image below the table is representative of data from image segmentation analysis. (c) 40x image of CD4 immunophenotyping overlaid with HIV gag RNA in HIV-infected PBMCs; Anti-CD4 antibody-Alexa Fluor® 488 (red), HIV gag RNA-Alexa Fluor® 546 (green), and DAPI (blue).