Figure 3. WT1-specific TCR-mediated cytocidal activity is not impaired by additional gene modification for expression of CCR2.

(A) Using standard ⁵¹Cr release assay, these double-transfected effector cells sufficiently lysed 1 µM WT1 peptide-loaded, but not unloaded, C1R-A24 cells that expressed HLA-A*2402. E/T ratio indicates effector∶target ratio. Error bar indicates SDs. (B) These double-transfected effector cells also sufficiently lysed HLA-A*2402⁺ LK79 cells, but not LK87 cells lacking the HLA-A*2402 molecule used as a negative control. (C) Double-transfected CD8⁺ T cells successfully expressed both cell-surface CCR2 and Vβ5.1, the germ-line component of the WT1-specific TCR β chain. CCR2/WT1-siTCR-CD8⁺ T cells indicates double-transfected CD8⁺ T cells.