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. 2013 Feb 18;8(2):e56820. doi: 10.1371/journal.pone.0056820

Figure 6. Positive cross-talk between CCR2 and WT1 epitope-responsive TCR signaling in double-transfected T cells.

Figure 6

(A) In double-transfected Jurkat/MA/CD8α/luc cells, CCL2 ligation to introduced CCR2 significantly augmented WT1-responsive luciferase production triggered by stimulation with 20 µM WT1 peptide-loaded C1R-A24 cells in a dose-dependent manner. In the absence of WT1 peptide, CCL2 ligation induced a low level of luciferase production. (B) In normal peripheral CD8+ T cells (n = 3) double-transduced to express both CCR2 and WT1-specific TCR, CCL2 ligation significantly enhanced WT1-responsive IFN-γ production in response to stimulation with 1 µM WT1 peptide-loaded C1R-A24 cells in a dose-dependent manner. (C) Similarly treated double-transfected normal effector cells (n = 3) showed a trend to increase WT1-responsive cytotoxic degranulation, but not to a significant degree. (D) Representative data for CD107a expression from 3 cases examined are shown. (B), (C) and (D) illustrate that cellular outputs triggered by TCR ligation with the WT1 epitope/HLA complex were augmented in the presence of CCL2. n.s. indicates not significant.