Fig. 5.
Average transduction by seven charged variants. (A) Lysozyme positions 83 and 119 were mutated to have positive (blue), neutral (yellow), or negative (red) charged sidechains. The effective gating ΔVG by each variant varied from 116 to −205 mV, with a value of −34 mV for the neutral N = 0 variant. (B) For all variants, ΔVG is nearly proportional to N, with a slope of 87 ± 2 mV per unit charge. Raw data (open squares) shifted up +34 mV (solid squares) results in a response symmetric around zero. Error bars indicate three standard deviations as determined from n (indicated in parentheses) different devices fabricated with each particular variant.