FIGURE 1.

WKYMVm-induced leukocyte transmigration and chemotaxis are enhanced in the absence of Fer kinase in vivo. Leukocyte emigration and chemotaxis were induced in vivo by placing WKYMVm (1 mM) in an agarose gel 350 μm from the vessel in the extravascular space. Data were observed in WT mice (open bars), Fer^DR/DR mice (solid bars), and bone marrow chimeric mice (WT donors into Fer^DR/DR recipients [EndotheliumFer^DR/DR, hatched bars] or Fer^DR/DR leukocytes into WT recipients [LeukocyteFer^DR/DR, vertical hatched bars]). (A) Illustrates total number of emigrated leukocytes observed in the extravascular space (cells/field of view) under control conditions (vehicle), or 60 min after WKYMVm gel placement. (B) Illustrates the number of extravasated cells in each 25-μm increment segment from the blood vessel toward the gel containing WKYMVm. Data are expressed as mean ± SEM, n = 4 (WT), n = 5 (Fer^DR/DR), n = 4 (Endothelium-Fer^DR/DR and Leukocyte-Fer^DR/DR chimeras), *p < 0.05 significant difference from WT mice group.