FIGURE 4.

Absence of Fer kinase alters the intracellular signaling pathways used by neutrophils to chemotax toward WKYMVm. In vitro neutrophil chemotaxis was determined using bone marrow–derived neutrophils from WT and Fer mutant (Fer^DR/DR) mice in the under-agarose gel assay. Chemotaxis was induced toward 1 μM WKYMVm (A) or 1.25 μM KC (B) and expressed as a percentage of the total number of cells moving toward the chemoattractants in untreated (UT) WT groups (taken as 100%; solid bars). The role of p38 MAPK or PI3K pathways in stimulus-induced chemotaxis was determined by pretreating the neutrophils with SB203580 (10 μM), SKF86002 (30 μM), SB239063 (10 μM), LY294002 (50 μM), wortmannin (100 nM), or a combination of wortmannin and SKF86002. Data are expressed as mean ± SEM, 10–16 plates (n = 9 mice). *p < 0.05 significant difference from untreated WT or Fer^DR/DR neutrophils.