FIGURE 6.

WKYMVm-induced PI3K activation is prolonged in neutrophils in the absence of Fer kinase. Bone marrow–derived neutrophils from WT and Fer^DR/DR mice were isolated and stimulated with WKYMVm (Pep; 1 μM) for 2–15 min. Lysates were prepared in triplicate, resolved by SDS-PAGE, immunoblotted with anti-pAkt Ab (Ser⁴⁷³), stripped, and reprobed with anti-Akt Ab. (A) Representative immunoblot of phosphorylated and total Akt in each condition. (B) Semiquantitative analysis of Akt phosphorylation determined by densitometric analysis of n = 4 immunoblots. Data are expressed as the mean ± SEM. Data from WT and Fer^DR/DR neutrophils are shown in open or solid bars, respectively. *p < 0.05 significant difference from control (Ct) of each strain, ^#p < 0.05 significant difference from WT neutrophils at same stimulation time condition.