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. 2013 Jan 16;3(2):85–92. doi: 10.7150/thno.5588

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Fig 3 — The electrophoresis results. (A) The effects of different elution buffers to elute the genome DNA from MNPs. The TE buffer was used in the 1^st, 2^nd and 3^rd lane while deionized water was used in the 4^th, 5^th and 6^th lane. primary culture of bacterium was loaded in the 1^st and 4^th well while the 10 time and 100 time diluted samples were loaded in the 2^nd and 5^th, 4^th and 6^th well respectively. (B) An in situ PCR for gyrB based on Fe₃O₄@SiO₂ MNPs: 1^st, 2^nd, 3^rd and 4^th lanes all are gyrB PCR products. (C) Lane 1 contains the results of in situ PCR; lane 2 contains the traditional PCR. In this study, the concentration of agrose gel was 1.0% in A and 1.5% in B and C.