Fig 4.

The chemiluminescent detection of Pseudomonas aeruginosa based on gyrB gene. (A) Effect of MNPs size on chemiluminescent intensity. MNPs-1: 400 nm sized probes modified MNPs, used to capture PCR products of gyrB gene. Control-1: the probes modified MNPs that captured the PCR products of bacterial universal gene. MNPs-2: 250 nm sized probes modified MNPs were used to capture PCR products of gyrB gene. Control-2: the probes modified MNPs that captured the PCR products of bacterial universal gene. (B) The effects of different binding sites of probes and different lengths of the target sequence on chemiluminescence intensity (Figure 2). 400 nm sized probes modified MNPswere used. gyrB: The gyrB probes were used to capture PCR products of gyrB gene. Control-1: The gyrB probes that captured PCR products of bacterial universal gene. ecfx-S: The ecfx probes were used to capture short PCR products of ecfx gene. ecfx-L: The ecfx probes were used to capture long PCR products of ecfx gene. Control-2: the ecfx probes that captured PCR products of bacterial universal gene.