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. 2013 Feb 19;7:22. doi: 10.3389/fncir.2013.00022

Figure 1.

Figure 1

Topographical distribution of GFP immunofluorescence in the NAc of Drd1a-EGFP mice. (A) Distribution of GFP in the NAc of Drd1a-EGFP BAC transgenic mice. Single confocal sections were stitched together as a single image. Scale bar: 500 μm. DStr, dorsal striatum; aca, anterior commissure; ICjM, islands of Calleja major; Tu, olfactory tubercles. (B) Schematic illustration of the different accumbal subterritories analyzed (see Figure A1). Cyan in the diagram represents distribution of GFP-expressing cells. A zone homogenously cyan means the GFP is homogenously distributed. Note that D1R-containing MSNs show a relative homogeneous distribution. Single scan confocal images stained for GFP (cyan) and DARPP-32 (red), a marker of MSNs in the dorsal caudomedial (GFP, B1; DARPP-32, B2; merge, B3) and ventral (GFP, B4; DARPP-32, B5; merge, B6) part of the shell of Drd1a-EGFP mice. Images shown are representative of all Drd1a-EGFP BAC transgenic mice analyzed (n = 4). Yellow asterisk identifies the D2R-expressing MSNs-poor zone. Scale bar: 200 μm.