Figure 11.

Regulation of ERK phosphorylation in the ventral NAc shell. (A) Double immunofluorescence for P-ERK (magenta) and GFP (green) in the NAc ventral shell of Drd2-EGFP mice treated with vehicle, SKF81297 (5 mg/kg), apomorphine (3 mg/kg), and raclopride (0.3 mg/kg). Scale bar: 100 μm. High magnification of the area delineated by the white dashed square. Yellow arrowheads indicate D2R-expressing MSNs that contain P-ERK. Images are single confocal sections. Scale bar: 50 μm. (B) P-ERK immunoreactivity (red) was detected together with DARPP-32 (blue) and GFP (green) immunoreactivities in the D2R-expressing MSNs-poor zone located in the ventral shell of Drd2-EGFP mice treated with SKF81297 (5 mg/kg) in a triple fluorescence analysis. Images are single confocal sections. Note that all P-ERK-positive cells co-localize with DARPP-32. Scale bar: 50 μm.