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. 2013 Feb 19;7:22. doi: 10.3389/fncir.2013.00022

Figure 8.

Figure 8

Distribution patterns of tyrosine hydroxylase (TH) and dopamine transporter (DAT) in the caudomedial NAc shell. (A) Distribution of TH (red) and GFP (cyan) fluorescence in the NAc of Drd2-EGFP and Drd3-Cre/Rosa26:loxP double transgenic mice. Yellow asterisk identifies the D2R-expressing MSNs-poor zone. Images are single confocal sections. Yellow arrowheads identify the bundle-shaped area. Scale bar: 250 μm. (B) Triple immunostaining for GFP (green), TH (red), and DAT (blue) allowed the identification of DA neurons on the unlesioned side of Drd2-EGFP mice. Note the lack of staining in the VTA on the lesioned side. Images are single confocal sections. Scale bar: 500 μm. (C) The same triple immunostaining performed at the caudomedial NAc shell level revealed the absence of DAT and a strong reduction of immunoreactive terminals in the D2R-expressing MSNs-poor zone (yellow asterisk). Images are single confocal sections. Scale bar: 250 μm.