Fig. 3. cic mutations cooperate with rbf1 mutation to promote proliferation and survival.

(A) Mosaic clones of cic mutant cells were generated in control or rbf1^120a mutant eye discs. Third instar eye imaginal discs were then incubated with EdU (red) to visualise S-phase cells and immunostained with anti-Atonal (blue). cic mutant clones are marked by the lack of GFP signal (green) and the position of the MF is marked by a yellow arrow. (B) Eye imaginal discs of the same genotypes as in A are shown. Anti-cleaved Caspase 3 (C3) was used to detect apoptotic cells (white). (C) Pupal eye discs (42 hours after pupal formation (APF)) of rbf1^120a mutant flies, flies with cic mutant clones and rbf1^120a mutant flies that contain cic mutant clones were immunostained with anti-Discs Large (red). (D) Scanning Electron Microscopy (SEM) images of Drosophila adult eyes entirely composed of rbf1^120a or cic single mutant cells and rbf1^120a cic mutant cells are presented.