Fig. 5. Cic mutant cells have a low level of Reactive Oxygen Species (ROS).

(A) Third instar eye discs containing mosaic clones of either rbf1 null mutant cells or cic mutant cells were stained with Dihydroethidium (Dhe) to monitor the levels of ROS. Note that the intensity of Dhe staining is weaker in cic mutant clones compared to the wild-type clones while it is relatively similar between the rbf1 mutant and control clones. (B) Mosaic clones of cic mutant cells were generated in rbf1^120a mutant flies. Third instar eye imaginal discs were stained with Dhe to monitor the level of ROS. Magnified views of the indicated areas (white box) are also presented.