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. 2012 Dec 19;2(2):227–237. doi: 10.1242/bio.20123699

Fig. 6. N-signalling affects Pa-cad and Pa-Wnt1 during germ band elongation.

Fig. 6.

(A) Wild type Pa-cad expression (bracket). (B) NRNAi embryos have a reduced GZ (outline, inset) completely devoid Pa-cad expression (*). (C) Wild type Pa-Wnt1 expression. (D) In NRNAi embryos there is no posterior Pa-Wnt1 expression (arrowhead), while the anterior segmental stripes remain unaffected (arrow; compare with C). (E–J) Effects of the N-inhibitor DAPT on posterior gene expression. (E–G) Pa-cad expression in the 0-hour control embryos (bracket, E) is unaffected in embryos cultured for 24 hours in DMSO control (bracket, F), but is reduced after 24 hours in DAPT culture (bracket, G). (H–J) Pa-Wnt1 is in segmental stripes (arrowhead) and a posterior arc (arrow) in embryos prior to culture (0-hour control, H) and after 24 hours in DMSO control culture, in which a new Pa-Wnt1 stripe forms in the anterior GZ (open arrowhead, I). (J) 24-hour DAPT cultured embryos show a reduction of the Pa-Wnt1 posterior arc from four to two cells in width and the new A4 stripe only partially formed (open arrowhead). T2: second thoracic segment; A3: third abdominal segment.