Protein phosphatase 1 and 2a inhibition (okadaic acid, OA) and PKA inhibition (PKI) abrogated the contractile difference between Sed and Ex myocytes. Summary data of Ca2+ transient amplitudes (A), Ca2+ transient RT50 (B), shortening amplitudes and (C), relengthening RT50 (D) in exercise (Ex-grey) and sedentary (Sed-black) myocytes. n=10-22 cells/4 hearts, *P< 0.05 vs corresponding Sed.