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Molecular characterization of the AP2 complex. (A) Western blot for GFP in lysates from wild-type cells (AX2) and GFP-expressing wild-type and apm2– cells. (B,C) Coomassie-stained SDS-PAGE gel of proteins immunoprecipitated with anti-GFP antibodies from (B) AX2, as well as AX2 expressing GFP-Ap2A1 and (C) apm2– and apm2– cells expressing GFP-Ap2A1. A list of all proteins uniquely identified by LC-MS/MS is in supplementary material Table S2.
