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. 2012 Dec 1;125(23):5790–5799. doi: 10.1242/jcs.110494

Fig. 5.

Fig. 5.

The effect of Rho-kinase inhibitor on F-actin bundle depletion and guided MT growth towards focal adhesions/vertices. Rat2 cells on triangular, fibronectin-coated islands were either untreated (see Fig. 3) or treated with ROCK inhibitor Y27632 (60 µM). (A) Cherry–actin distribution in a representative experimental cell shows depletion of F-actin bundles. (B) The reconstructed MT growth trajectories (green tracks) from the same cell; triangle indicates cell boundary. (C) F-actin and (D) focal adhesion (vinculin) distribution in cells treated with Y27632. Scale bar: 10 µm and is the same for all images. (E) The probability distributions of the MT growth directions; red, untreated; blue, Y27632; black, random/unguided. Data for Y27632-treated cells is based on 367 trajectories from five cells. (F) Quantification of the extent of F-actin bundle depletion. ROCK inhibitor depletes interior F-actin bundles and results in random/unguided MT growth. A two-sample t-test shows that the probability (at θ = 0°) for cells treated with Y27632 is statistically similar to the unguided case.