Western blots of serum samples from control and immunized BALB/c, C3H/HeN and C57BL/6 mice reacted with MoPn EB. Lane 1) MW standards; lane 2) control monoclonal antibody to MOMP. Serum samples from mice immunized with: lane 3) live EB, i.n. at 60 days post-immunization (d.p.i.); lane 4) live EB, i.n. at 120 d.p.i.; lane 5) live EB, i.n. at 180 d.p.i.; lane 6) live EB, i.vag. at 60 d.p.i.; lane 7) live EB, i.vag. at 120 d.p.i.; lane 8) live EB, i.vag. at 180 d.p.i.; lane 9) UV-EB+CpG+Montanide at 60 d.p.i.; lane 10) UV-EB+CpG+Montanide at 120 d.p.i.; lane 11) UV-EB+CpG+Montanide at 180 d.p.i.; lane 12) UV-EB+Alum at 60 d.p.i.; lane 13) UV-EB+Alumat 120 d.p.i.; lane 14) UV-EB+Alumat 180 d.p.i.; lane 15) Ova+CpG+Montanide+Alumat 60 d.p.i.; lane 16) Ova+CpG+Montanide+Alumat 120 d.p.i.; lane 17) Ova+CpG+Montanide+Alumat 180 d.p.i.; lane 18)MEM-0 at 60 d.p.i.; lane 19) MEM-0 at 120 d.p.i.; lane 20) MEM-0 at 180 d.p.i.; lane 21) pre-immunization serum.