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In vitro, (D)-DT-2 selectively inhibits PKG Iα and PKG Iβ but not PKG II or PKA. Indicated concentrations of (D)-DT-2 were added to purified (A) PKG Iα, (B) PKG Iβ, (C) PKG II and (D) PKAc (2 nM each). PKGs were stimulated by addition of 5 µM cGMP (PKAc is catalytically active). Kinase activity was determined by phosphorylation of Vasptide (for PKGs) or Kemptide (for PKAc), both used at 20 µM.
