Figure 2.

Quantitative characterization of PKG expression in RMC and NRVM. Indicated concentrations of purified PKG Iα/β and cells were loaded on the gel and processed for Western blotting. Blots were scanned using SilverFast software and analysed densitometrically by NIH Image J software for uncalibrated optical density. Cell volumes calculated from confocal 3D stacks (representative microscopy images are shown in B and C. Scale bars, 10 µM) were 6.3 pL for RMC and 3.5 pL for NRCM. In both cell types, only PKG Iα is expressed in concentrations of 0.36 ± 0.03 µM for RMC and 0.30 ± 0.02 µM for NRVM.