Figure 4.

(D)-DT-2 does not inhibit PKG activity in intact human platelets. 100 µL of washed human platelets (2 × 10⁸ cells·mL⁻¹) were pre-incubated with indicated concentrations of (D)-DT-2 for 30 min (A), or with 100 µM of (D)-DT-2 for indicated time points (B). PKG was activated by DEA-NO (1 µM, 1 min). Samples were processed for Western blotting and PKG activity was evaluated by VASP-Ser²³⁹ (P-VASP) and PDE5-Ser⁹² (PDE5) phosphorylation. PDE5 expression served as a loading control. The results are representative of four independent experiments.