Figure 5.

Vestibular ganglion of adult rats expresses H₄ receptor mRNA. (A) Structure of H₄ receptor gene with lengths of exons (rectangles) and introns shown in base pairs. Arrows represent orientation and approximate locations of primers used in our RT-PCR experiments. A sequence specific primer was used to reverse transcribe RNA transcript, F1/R1 and F2/R2 were used for PCR and reamplification, respectively. (B) The figure shows a representative example of PCR products obtained for H₄ receptors (477 bp) in the spleen (S) and vestibular ganglia (VG). GAPDH (502 bp): positive control to attest the success of the PCR reaction. RT (–): negative RT control where water has been substituted for reverse transcriptase. Size of the PCR products if genomic DNA was present in our samples as a template for amplification should be 2500 bp (GAPDH) and larger than 15 kb (H₄ receptor), respectively. No band with this predicted size was observed.