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. 2012 Dec 27;41(4):2683–2688. doi: 10.1093/nar/gks1304

Figure 2.

Figure 2.

Resonance assignments of s2 and s4 in K+ solution at 25°C. (a and c) Imino protons of s2 and s4, respectively, were assigned by site-specific 2% 15N-labeling. (b and d) H8 protons of s2 and s4, respectively, were assigned by through-bond correlations between imino and H8 protons via 13C5 at natural abundance, using long-range J couplings shown as insert in (d).