Figure 4.

HIV-1 integrase inhibition disintegration assays. Lanes ‘N’ and ‘P’ are controls without and with HIV-1 integrase alone, respectively. Other lanes are inhibition assays with added inhibitors as indicated, where dic is the Dickerson d(CGCGAATTCGCG) dodecamer and telo is the human telomeric sequence d[TT(GGGTTA)₃GGGA]. The concentrations of the enzyme, substrate and inhibitors were 0.6, 0.2 and 3.0 µM, respectively. The DNA substrate (dumbbell DB-Y1) and disintegration product (see ‘Methods’ section) are monitored as bands of 38 nt (top band) and 14 nt (bottom band), respectively (33,34).