Table 1.

Sequences of NCp7 mutants studied in the present work (the wild-type sequence corresponds to the HIV-1 from pNL4-3)

NC wild-type	M Q K G N F R N Q R K T V K CF NCG K E GHI A K NC R A P R K R G CW KCG K E GHQ M K DC T E R Q A N
Finger swap mutants (36):
NC 1/1	M Q K G N F R N Q R K T V K CF NCG K E GHI A K NC R A P R K R G CF NCG K E GHI AKNC T E R Q A N
NC 2/2	M Q K G N F R N Q R K T V K CW KCG K E GHQ MKDC R A P R K R G CW KCG K E GHQ M K DC T E R Q A N
NC 2/1	M Q K G N F R N Q R K T V K CW KCG K E GHQ MKDC R A P R K R G CF NCG K E GHI AKNC T E R Q A N
Zn coordination mutants (24,47):
H23C	M Q K G N F R N Q R K T V K CF NCG K E GCI A K NC R A P R K R G CW KCG K E GHQ M K DC T E R Q A N
H44C	M Q K G N F R N Q R K T V K CF NCG K E GHI A K NC R A P R K R G CW KCG K E GCQ M K DC T E R Q A N
H23C/H44C	M Q K G N F R N Q R K T V K CF NCG K E GCI A K NC R A P R K R G CW KCG K E GCQ M K DC T E R Q A N
SSHS/SSHS	M Q K G N F R N Q R K T V K SF NSG K E GHI A K NS R A P R K R G SW KSG K E GHQ M K DS T E R Q A N
Aromatic and basic residue mutants (34,49):
F16A	M Q K G N F R N Q R K T V K CANCG K E GHI A K NC R A P R K R G CW KCG K E GHQ M K DC T E R Q A N
W37A	M Q K G N F R N Q R K T V K CF NCG K E GHI A K NC R A P R K R G CAKCG K E GHQ M K DC T E R Q A N
F16A/W37A	M Q K G N F R N Q R K T V K CANCG K E GHI A K NC R A P R K R G CAKCG K E GHQ M K DC T E R Q A N
F16W	M Q K G N F R N Q R K T V K CWNCG K E GHI A K NC R A P R K R G CW KCG K E GHQ M K DC T E R Q A N
W37F	M Q K G N F R N Q R K T V K CF NCG K E GHI A K NC R A P R K R G CFKCG K E GHQ M K DC T E R Q A N
F16W/W37F	M Q K G N F R N Q R K T V K CWNCG K E GHI A K NC R A P R K R G CFKCG K E GHQ M K DC T E R Q A N
Nterm (62):	M Q A G N F A N Q A A I I ACF NCG K E GHI A K NC R A P R K R G CW KCG K E GHQ M K DC T E R Q A N

The residues of the Zn finger motifs are underlined with the Zn binding Cys and His residues in bold. The mutations are shown in italics.