Figure 3.

Tandem CAR-E containing dinucleotide substitutions is non-functional in RNA export. (A) Schematic of CMV-β-globin cDNA construct showing sequences of the CAR-E and each of the mutants that were inserted into the cDNA. Mutated nucleotides are shown in lower case. (B) Western analysis at 24 h (Day 1) or 45 h (Day 2) after transient transfection of the indicated constructs into HeLa cells. HA-β-globin and tubulin (loading control) are indicated. (C) Nucleocytoplasmic distribution of CAR-E_m1-5 transcripts was determined by FISH. DAPI staining was used to identify the nucleus. Scale bar: 10 µm.