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. 2012 Dec 25;41(4):e58. doi: 10.1093/nar/gks1324

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

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Figure 6. — Mitochondrial DNA topoisomers are associated with RNA in 2D-IMAGE profiles. (A) 2D-IMAGE profile of wild-type MEF DNA without RNAse treatment. New topoisomers relative to those in Figure 3 are assigned letters. (B) RNase H treatment, which digests RNA:DNA hybrids, reveals the vertical spikes of DNA that are sensitive to S1 nuclease (23–25). Molecules that decrease on digestion are indicated with dashed arrows, whereas those that increase are indicated with solid arrows. Numbers correspond to topoisomers in Figure 3. (C) RNase A treatment, which digests heterogeneous RNA after U and C bases, reveals the typical 2D-IMAGE pattern shown in Figure 2. Dashed arrows indicate a reduction in signal, and solid arrows indicate increased signal. (D) Quantitation of change in abundance of topoisomers shown in panels B and C relative to untreated.