Figure 4.

Schematic representation of the three pairs of ZFNs targeting IRS2, met and sim1a. Only the six-finger constructs are shown. Finger number was reduced by progressively removing fingers from the N-terminus of these constructs. The positions of two-finger modules (GANNAG) described herein or fingers from other archives [GRNNCG (50) and single fingers (41)] within these ZFAs are indicated, as is the position of the THPRAPIPKP linker that allows a single base pair (underlined lowercase base) to be skipped between intervening modules (65). The efficiency of lesion generation in zebrafish embryos by these ZFAs as a function of the number of fingers is indicated in the chart, where red highlights indicate the high toxicity of the met ZFNs.