Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Jan 8;41(4):2255–2266. doi: 10.1093/nar/gks1454

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — The COX4I2 promoter ORE binds three proteins, RBPJ, CXXC5 and CHCHD2. (A) The conserved 13-bp ORE in human COX4I2 promoter upstream of exon I is shown, with coordinates from the translation start site located in exon II. Bases shown in boldface form the consensus RBPJ binding site. (B) HeLa cell yeast one-hybrid analysis was performed to identify binding proteins of the ORE. Three proteins were identified that specifically bound the bait sequence. (C) DNA binding assay was performed in 293 cells to confirm binding to the ORE of the proteins identified in the one-hybrid screen. (D) HIF-1α fails to bind the ORE in the DNA binding assay described in the ‘Materials and Methods’ section.