Figure 1.

Interacting partners of the 2 micron circle partitioning proteins Rep1 and Rep2. The two types of constructs used for TAP tagging, with the tagged REP locus under control of the GAL promoter (A) or the native promoter (B), are schematically diagrammed. Identification of tryptic peptides by mass spectrometry was performed on the unfractionated ensemble of proteins enriched by a two-step affinity purification of tagged Rep1 or Rep2 (see Materials and Methods). The list, except for Rep1, Rep2 and Raf1, comprises a subset of interacting partners that are constituents of, or are associated with, chromatin remodeling complexes. Sequence count refers to the number of distinct peptides, spectral count to the number of total peptides and sequence coverage to the percentage of amino acids of the full-length protein represented by the sequenced peptides. Those cases where more than one value is shown for each of these parameters refer to separate experiments. This analysis used strains MJY4013 to MJY4025 listed in Supplementary Table S1.